Template:IONIS Paris/Notebook/07-07-15

PCR pDawn BioBricks

Results of the latest transformation

Aim: first step for pDawn amplification and mutagenesis

No colonies have grown

Electrophoresis

Gel : 1,2 g agarose + 120 mL of TAE 1x + 150 µL of BET
Migration of the Gibson product

Expected results

Results

Soft bands: it means that some reactions occured

7 July 15