Template:IONIS Paris/Notebook/08-07-15

PCR pDawn BioBricks

Results of the latest transformation

Aim: first step for pDawn amplification and mutagenesis

After 48h, 2 white shapeless colonies have grown --> Liquid culture (20mL of LB + 20µl of Cam)

Production of competent cells (1st step)

Culture of E.coli DH5 alpha from NEB on a non-selective agar plate with a control (plate without bacteria)

8 July 15