Template:IONIS Paris/Notebook/09-07-15

Production of competent cells (next steps)

Results of the first step: bacterial amplification

Good bacterial amplification --> miniprep with a MOBio kit (20 ml into 2 columns, final elution with 50 µl for each)
The first PCR to amplify pDawn III has been made by our advisor Samuel Juillot.

Second step

Bacteria have grown on the plate; no bacteria into the control
Pre-liquid culture into an Erlenmeyer without selective condition using an isolated colony from the plate; with a control (Erlenmeyer without bacteria)

9 July 15