Template:IONIS Paris/Notebook/10-06-15

Miniprep

Protocol from QIAgen, UltraClean® Standard Mini plasmid Prep Kit

  • BBa_I712074
  • BBa_K124003

Glycerol stock

Stock of BBa_I712074 and BBa_K124003 (cf protocol for glycerol stock), -80°C

Electrophoresis

Migration of purified BBa_I712074 and BBa_K124003

Expected results

Expected results

Results

Results

Bands do not correspond exactly to expected weights (supercoiled)

Plate preparation

3 plates: 20 mL of LB agar + 20 µL of Cam

Liquid culture

Double click tube: 3 mL of LB broth + 3 µL of antibiotic

Tube preparation
Tube Antibiotic
BBa_I712074
Cam
BBa_K124003
Cam
Control Negative
Cam

Survival checking of bacteria after a night spent into a -20°C freezer
Erlenmeyer: culture of bacteria transformed with pSB1C3 coming from glycerol stock; 20 mL of LB broth + 20 µL of Cam


10 June 15