Template:IONIS Paris/Notebook/10-06-15
Miniprep
Protocol from QIAgen, UltraClean® Standard Mini plasmid Prep Kit
- BBa_I712074
- BBa_K124003
Glycerol stock
Stock of BBa_I712074 and BBa_K124003 (cf protocol for glycerol stock), -80°C
Electrophoresis
Migration of purified BBa_I712074 and BBa_K124003
Expected results
Results
Bands do not correspond exactly to expected weights (supercoiled)
Plate preparation
3 plates: 20 mL of LB agar + 20 µL of Cam
Liquid culture
Double click tube: 3 mL of LB broth + 3 µL of antibiotic
Tube | Antibiotic |
---|---|
BBa_I712074 | |
BBa_K124003 | |
Control Negative | |
Survival checking of bacteria after a night spent into a -20°C freezer
Erlenmeyer: culture of bacteria transformed with pSB1C3 coming from glycerol stock; 20 mL of LB broth + 20 µL of Cam
10 June 15