Template:IONIS Paris/Notebook/11-06-15

Result from 10.06.15

Culture liquid

pSB1C3 culture liquid

Expected results






pSB1C3: medium became red (RFP) and highly cloudy

BBa_I712074 and BBa_K124003 culture liquid

Expected results






BBa_I712074 and BBa_K124003: cloudy, no contamination in CT-, it still alive bacteria

Miniprep

Protocol from MOBIO Kit

  • pSB1C3: 20 ml into 4 columns, eluted with 30 µl (pooled together)

Electrophoresis

Gel: 1,2 g of agarose + 120 ml of TAE 1x + 150 µl of BET
Migration of purified pSB1C3

Expected results

Expected results

Results

Results

Quantification of pSB1C3 by Image J = 35 – 40 ng.µL-1
4 µg (min) of pSB1C3 are requiring for digestion of the plasmid to make a Gibson reaction

  • 35 ng x 115 µl (remaining) = 4,025 µg all the remaining 115 µL of pSB1C3 should be digested


    • 11 June 15