Template:IONIS Paris/Notebook/17-07-15
Results of bacterial transformation
A total of 12 colonies in both plates containing transformed bacteria
No colony into the negative control
“Biofilm” into the positive control, bacteria are alive and competent
Gel extraction from the cut band of the 16th of July
We used the kit from QIAgen to extract the DNA from the gel
Final elution into 50 µL
Digestion of Gibson product (VVD-YC155)
| Tube | Gibson purified |
|---|---|
| Water | 12 µL |
| Buffer 2.1 | 2 µL |
| Plasmid | 5 µL |
| Enzyme EcoRI | 0,5 µL |
| Enzyme PstI | 0,5 µL |
37°C, 60 min
17 July 15