Template:IONIS Paris/Notebook/17-09-15
Miniprep of liquid culture (pSB1C3-Nanoluc)
Digestion of miniprep
| Tube | pSB1C3 |
|---|---|
| H2O MQ | 4 µL |
| Buffer 2.1 | 1 µL |
| DNA | 3 µL |
| EcoRI | 1 µL |
| PstI | 1 µL |
37°C, 1h
Expected results
Results
No profile of double digestion for pSB1C3-nanoluc. The third sample looks like a simple digestion PCR checking
- Biobrick quantification for submission:
- Holin/Endolysin reverse: 115 ng/µl
- CCDB reverse: 73 ng/µl
- HOKD reverse: 10 ng/µl
- VVD-YC: 38 ng/µl
- VVD-YN: 37 ng/µl
PCR pSB1C3-NanoLuc
| Tube | pSB1C3-NanoLuc (x5) |
|---|---|
| MQ Water | 40 µL |
| RB Buffer | 5 µL |
| Mg2+ | 1,5 µL |
| dNTP 10 µM | 1 µL |
| Primer pSB1C3 Fwd | 1 µL |
| Primer pSB1C3 Rev | 1 µL |
| DNA | 1 µL |
| Taq Pol | 0,5 µL |
37°C, 1h
iGEM TAQ
17 Sept. 15