Template:NYMU-2015project-wetlab-Defensin

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Project

Cure

Overview

Our goal is to kill P. infestans without harming the potatoes and environment. Fungicides used to kill P. infestans nowadays contain lots of heavy metal, such as Cu2+ that will do harm to both the plant and environment. At first we try to use some antimicrobial peptides; however this peptide will not only kill P. infestans but do harm to potatoes as well. Therefore, we try to look for the defensin that can weaken or even kill P. infestans from other plants. Although there are other chemicals that might be effective in inhibiting the growth of P. infestans, such as 2, 6-dichlorobenzonitrile, but some of these may also do harm to bacteria itself. Based on the above reasons, we finally choose lm-defensin, a defensing from maca that can effectively weaken and inhibit the growth of P. infestans

Circuit design

To purify defensin, we put a His tag on the N-terminal of the defensin. Also to produce lots of defensin, we choose T7 promoter and E.coli BL21 (DE3) which is used to express huge amount of proteins.