Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10

Transformation

pdark natural transformation

In the evening of Sep. 11

6th liquid segregation of pdark-HR

pcpcG2 electro-transformation

In the evening of Sep. 11

5th liquid segregation of pcpcG2-HR

pdark-GFP electro-transformation

Sep. 8

We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation