Template:SJTU-BioX-Shanghai/Notebook/Transformation/w7

Transformation

pdark natural transformation

In the evening of Aug 18

We picked 4 colonies (marked with 1,2,3 & 4) from the pdark-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation

In the evening of Aug. 20

We scraped the grown cell from the plate, which is 1,3 & 4 to do colony PCR, 1st liquid segregation and solid storage

pre-experiment of pdark-HR

In the evening of Aug. 20

Inoculated 16 wildtype & 16 pdark-HR (use pdark-HR from electro-transformation), added 11g/L or 22g/L NaCl & retinene at the beginning

In the evening of Aug. 22

Induced with darkness, took the first 4 samples at 9pm

In the evening of Aug. 23

Cultivated with white light, took the second 4 samples at 9pm.