Template:Team:Groningen/CONTENT/EXPERIMENTS/Digestion BBa K823023 and BBa K823024
00:00, 2 June 2015 - 00:00, 2 June 2015
The backbones BBa_K823023 and BBa_K823024 were digested and ligated with tasA by the following steps.
#
Component
Amount
1
Mastermix
28 µL
DNA (BBa_K823023)
2 µL
2
Mastermix
28 µL
DNA (BBa_K823024)
2 µL
Digestion mixtures.
The samples were digested for 2 hours at 37 °C. Afterwards they were loaded on 1% agarose gel with SERVA DNA stain G.