Template:Team:Groningen/CONTENT/EXPERIMENTS/Ligation tasA, Psalt in K823023 (t46)
00:00, 16 July 2015 - 00:00, 16 July 2015
The samples 1, 2 and 3 from the digestion step were ligated using the following amounts.
Component
Amount
DNA 1 (Psalt)
5 µL
DNA 2 (T2)
5 µL
DNA 3 (K823023)
2.5 µL
T4 ligase
0.5 µL
10x buffer
2 µL
\( \mathrm{H_2O}\)
5 µL
Components ligation sample.
The sample was ligated at room temperature for 30 minutes.