Template:Team:Groningen/CONTENT/EXPERIMENTS/PCR a26
00:00, 2 September 2015 - 00:00, 2 September 2015
PCR of abrB KO from BD1807 strains and Veening DNA.
Sequence primer forward:
Type of primer
sequence
Sequence primer forward
GAAGTGTACGATGCTTACC
Sequence primer reverse
CGGTAGTTTCCAAGACATTACTG
Recombination primer forward
CTGCAGCGGCCGCTACTAGTAGCGGAAGATACGAGGCAAAC
Recombination primer reverse
GAATTCGCGGCCGCTTCTAGAGAGACGATCCGCGTATTTCCC
Primer sequences
The was primer sequenced for sequencing the pcr product.
Recombination primers for creating abrB knockout in a biobrick.
PCR failed, as no bands were found on agarose gel.