Template:Team:Groningen/CONTENT/EXPERIMENTS/Psalt Colony PCR (zp34)

00:00, 18 August 2015 - 00:00, 18 August 2015

White colonies from the plate with transformed E. coli (pSB1C3 + Psalt) were checked for the right insert with the primers seen in table 1. After PCR samples were loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results show the correct insert.

Primer

Sequence

VR

attaccgcctttgagtgagc

VF2

tgccacctgacgtctaagaa

Primer sequences used in colony PCR.

Marieke Mulder