The following constructs were digested. After digestion DNA was purified using gel purification on a 1% agarose gel, run at 100V and cut out. Afterwards the samples were purified using a PCR purification kit.
Template:Team:Groningen/CONTENT/EXPERIMENTS/Psalt Digestion (zp62)
00:00, 26 August 2015 - 00:00, 26 August 2015
Constructs
Restriction enzymes
Psalt in pSB1C3
SpeI and PstI
RBS + GFP in pSB1C3
XbaI and PstI
Psalt + tasA in pSB1C3
EcoRI and PstI
Psalt + bslA in pSB1C3
EcoRI and PstI
BBa_K823023
EcoRI and PstI
Digestion samples.