This was done by growing B. subtilis ComI in LB + chloramphenicol for four hours. Then different salt concentrations were added (0.1M, 0.5M and 1M). This was incubated for 1 hour at 37°C. Afterwards cells were fixated in 1,25% agarose in PBS. Fluorescent microscopy did not show any differences between salt concentrations.
Template:Team:Groningen/CONTENT/EXPERIMENTS/Psalt GFP Validation (pz81)
00:00, 1 September 2015 - 00:00, 1 September 2015