Template:Team:Groningen/CONTENT/EXPERIMENTS/Psalt PCR (zp29)

00:00, 16 August 2015 - 00:00, 16 August 2015

PCR was performed with the forward and the new reverse primer for Psalt (figure 1) and a annealing temperature of 55°C and extention time of 60 seconds. PCR product was checked and purified on 1% agarose gel with 1:50000 SERVA DNA Stain G. Gel results show a band at approximately 600bp.

Primer

Sequence

salt primer 2 for

CCAGTGAATTCGCGGCCGCTTCTAGAGGTCTGAATTTAAAGAGATTGGTTTTAAACC

salt primer 2 rev

GGGATCCTGCAGCGGCCGCTACTAGTAGATCCTGCTCCTATAAAGGCTACTTTCTTT

Primer sequences

Marieke Mulder