Template:Team:Groningen/CONTENT/EXPERIMENTS/Psalt PCR (zp5)

00:00, 30 June 2015 - 00:00, 30 June 2015

PCR was performed with the forward and reverse primer for Psalt (figure 1) and a annealing temperature of 40°C. PCR product was checked and purified on 1% agarose gel with 1:50000 SERVA DNA Stain G. Gel results show a band at approximately 600bp.

Primer

Sequence

Forward

GCGGGGGAATTCGCGGCCGCTTCTAGAGGTCTGAATTTAAAGAGATTG

Reverse

GAGCTTTACTAGTAGCGGCCGCTGCAGGATCCTGCTCCTATAAAGGCTAC

Primer sequences

Marieke Mulder