Template:Team:Groningen/CONTENT/EXPERIMENTS/Tested in CFR py3
10:45, 07 Augustus 2015 - 00:00, 00 Month 2015
Approximately 15 mL MSgg agar with ampicillin was poured in one compartment of the continuous flow reactor for submerged biofilm growth.
In the meanwhile 10 µL of Bacillus subtilis py79 was added to 2 mL LB.
Bacillus subtilis py79 resuspended in PBS was placed in the other compartment of the continuous flow reactor.
After running the loaded continuous flow reactor, it appeared that the air in the system was not refreshed once it was on. Furthermore, Bacillus subtilis PY79 growth was not on the carrier, but in the medium.