Template:Team:Groningen/CONTENT/EXPERIMENTS/Transformation of tasA with Psalt (t78)
00:00, 17 August 2015 - 00:00, 17 August 2015
The ligations of tasA with the salt promoter were used to transform E. coli cells from NEB. 10 μL ligation mix was used for each transformation. The transformations were plated on LB plates with appropriate antibiotics. Ligations were performed according to manufacturer with an ratio 1:4 (Backbone:insert).