Template:Team:Groningen/CONTENT/EXPERIMENTS/sinR Transformation 3610 comI si9
10:00, 26 August 2015 - 00:00, 26 August 2015
Bacillus subtilis 3610 delta comI was added to LB at 10.00.
Afterwards the optical density of Bacillus subtilis 3610 delta comI was was measured: 0.120.
At 15:24 Bacillus subtilis 3610 delta comI was added to MC and the mixture was placed in the 37°C incubator.
At 20:24 1 µL of SinR plasmid was added to the MC mixture, which was placed back in the 37°C incubator.
At 23:00 the transformed Bacillus subtilis 3610 delta comI was plated.
00:00, 27 August 2015 - 00:00, 27 August 2015
Transformation succeeded, as there were colonies on the plate.