Template:Team:Groningen/CONTENT/EXPERIMENTS/sinR Transformation JH642 si16
10:00, 27 August 2015 - 00:00, 27 August 2015
Bacillus subtilis JH642 was added to LB.
Standard competence medium was prepared. 10 µL of phenylalanine was added to Bacillus subtilis JH642 mixture.
Bacillus subtilis JH642 was added to MC and the mixture was placed in the 37°C incubator at 13:00.
At 18:00 1 µL of SinR plasmid was added to the MC mixture, which was placed back in the 37°C incubator.
At 21:00 200µL transformed Bacillus subtilis JH642 was plated on spectinomycin plate.
00:00, 28 August 2015 - 00:00, 28 August 2015
Transformation succeeded, as there were colonies on the plate.