Template:Team:Groningen/CONTENT/EXPERIMENTS/sinR Transformation Natto si17
13:08, 29 August 2015 - 00:00, 29 August 2015
Bacillus subtilis natto was added to LB .
Bacillus subtilis natto was added to MC and the mixture was placed in the 37°C incubator.
At 18:00 1 µL of SinR plasmid was added to the MC mixture, which was placed back in the 37°C incubator.
Plated transformed Bacillus subtilis natto on LB with spectinomycin.
13:08, 30 August 2015 - 00:00, 29 August 2015
Transformation succeeded, as colonies were present on the plate.