Template:Team:Groningen/CONTENT/EXPERIMENTS/sinR Transformation PY79 si2
15:48, 24 August 2015 - 24:00, 24 August 2015
Standard competent medium (MC) was placed in the 37°C incubator. The OD was measured at 20:30, but Bacillus subtilis PY79 needed more time to grow. 1 µL of SinR DNA was added to 200 µL of MC with Bacillus subtilis PY79. The culture was plated at 24:00.
18:00, 25 August 2015 - 24:00, 25 August 2015
The transformation had failed as there were no colonies on the plate.