Template:Team:Groningen/CONTENT/EXPERIMENTS/sinR Transformation PY79 si5
14:37, 25 August 2015 - 00:00, 25 August 2015
MC was placed in the 37°C incubator. The OD was measured at 14:37, Bacillus subtilis PY79 had not grown enough. OD was measured to be 0.021 after 10x dilution with demiwater. 1 µL of SinR DNA was added to 200 µL of MC with Bacillus subtilis PY79 at 19:55. The cultures were not plated until the following morning.
00:00, 26 August 2015 - 00:00, 26 August 2015
Experiment needed to be repeated, since the plating should have occured two hours after adding DNA.