Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA, Pveg and Psalt Digestion (t56)
09:30, 28 July 2015 - 16:00, 28 July 2015
The digestion of the T2 sample of 10 July with two promoters was performed, both with the Pveg promoter from the iGEM distribution kit 2015 (plate 1, 20G) and the Psalt promoter. For the promoters EcoRI and SpeI were used as restriction enzymes, and XbaI and EcoRI for the backbone. The following samples were made.
#
Component
Amount
1
T2 DNA
5 µL
10x buffer
2 µL
EcoRI
1 µL
XbaI
1 µL
\( \mathrm{H_2O}\)
11 µL
2
Pveg DNA
10 µL
10x buffer
2 µL
EcoRI
1 µL
SpeI
1 µL
\( \mathrm{H_2O}\)
6 µL
4
Psalt DNA
5 µL
10x buffer
2 µL
EcoRI
1 µL
SpeI
1 µL
\( \mathrm{H_2O}\)
11 µL
Components digestion samples.
The samples were digested at 37 °C for 2 hours.