Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Colony PCR (t66)
00:00, 9 August 2015 - 00:00, 9 August 2015
The transformation of 7 August was checked. Some white colonies were visible. With these colonies a colony PCR was carried out. After the PCR the samples were loaded on a 1% agarose gel and it was seen that sample 3 and 7 possibly have the insert. Cultures were made of sample 3 and 7 by putting some of the bacteria in a tube with 3 mL LB+cm. The tubes were put in the shaking incubator at 37 °C with 200 rpm.