Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Digestion (t61)
13:22, 6 August 2015 - 18:40, 6 August 2015
PliaG was digested for ligation with T2. Restriction enzymes were used that create a scar. For the digestion the restriction enzymes EcoRI and SpeI were used for the promoter and XbaI and EcoRI for T2. Below are listed the amounts that were added of each component.
#
Component
Amount
1
PliaG DNA
5 µL
10x buffer
2 µL
EcoRI
1 µL
SpeI
1 µL
\( \mathrm{H_2O}\)
11 µL
2
T2 DNA
5 µL
10x buffer
2 µL
XbaI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
11 µL
Components for digestion.
The sample was digested for 2 hours at 37 °C.