Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Digestion (t69)
10:30, 13 August 2015 - 20:15, 13 August 2015
The digestion was carried out using for the promoter PliaG, the restriction enzymes SpeI and PstI, and for tasA (T2), the enzymes XbaI and PstI. The following digestion samples were prepared.
#
Component
Amount
1
PliaG DNA
10 µL
10x buffer (2.1)
2 µL
SpeI
1 µL
PstI
1 µL
\( \mathrm{H_2O}\)
6 µL
2
T2 DNA
10 µL
10x buffer (2.1)
2 µL
XbaI
1 µL
PstI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.
The samples were incubated at 37 °C for 3.5 hours.