Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Heat shock transformation (t47)
00:00, 16 July 2015 - 00:00, 16 July 2015
Afterwards the heat shock transformation from the protocol book was performed. For this, 10 µL competent NEB cells with 10 µL ligation product was used. The resulting cells were plated on LB+amp plates and grown overnight at 37 °C.
00:00, 17 July 2015 - 00:00, 17 July 2015
The transformation of the ligation product of the salt promoter with tasA was checked. On the plates a few white colonies were seen. Miniprep and colony PCR were performed to check whether the insert size was the right one. The colony PCR showed possible tasA with Psalt insert in one sample. This colony was grown overnight in LB+amp.