Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA K823023 Ligation (t22)

00:00, 3 June 2015 - 00:00, 3 June 2015

For the ligation of tasA in BBa_K823023, the following components were used.

Component

Amount

Vector DNA (K823023)

2 µL

Insert DNA (tasA)

2 µL

10x buffer

2 µL

T4 ligase

1 µL

\( \mathrm{H_2O}\)

13 µL

Components used for ligation.

The ligation mixture was left 2 hours at room temperature.

Harm Ruesink