Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Ligation product purification (t21)

00:00, 2 June 2015 - 00:00, 2 June 2015

The bands were cut out of the gel and purified with the PCR purification kit.

Sample

Mass gel(mg)

Binding buffer (µL)

tasA

77

144

K823023

181

362

K823024

133

266

Amounts of DNA and binding buffer added.

The samples were eluted with 20 µL elution buffer and stored at -20 °C as:

tasA DNA ligate

K823023 ligate

K823024 ligate

Harm Ruesink