Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA PCR (t9)
00:00, 19 May 2015 - 00:00, 19 May 2015
The following PCR was performed.
Compound
Amount
5x buffer
14 µL
dNTP MM (2 mM)
7 µL
Phusion polymerase
0.7 µL
\( \mathrm{H_2O}\)
47.6 µL
Mastermix for PCR 4.
Compound
Amount
Mastermix PCR 4
30 µL
Template DNA
0.3 µL
Each primer
0.3 µL
Components per sample.
#
Primer 1
Primer 2
Template DNA
1
5 tasA for
6 tasA rev
tasA DNA 1
2
5 tasA for
6 tasA rev
tasA DNA 2
Components per sample.
#
Step
Temperature
Time
1
Initial denaturation
98 °C
3:00
2
Denaturation
98 °C
0:30
3
Annealing
55 °C
0:30
4
Extension
72 °C
0:30
5
Go back to step 2 (30x)
6
Final extension
72 °C
10:00
PCR Thermocycle.
The product was stored in the freezer at -20 °C.