Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA PliaG Ligation (t64)
13:22, 6 August 2015 - 18:40, 6 August 2015
The ligation was carried out overnight at room temperature using the following components.
Component
Amount
PliaG DNA
5 µL
T2 DNA
10 µL
10x T4 buffer
2 µL
T4 ligase
0.5 µL
\( \mathrm{H_2O}\)
2.5 µL
Components for ligation of PliaG with T2.