Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Sequencing (t59)

00:00, 30 July 2015 - 00:00, 30 July 2015

The transformation of 29 July was checked. No colonies were visible on the LB+cm plate. T2 was send to Macrogen for sequencing.

Component

Amount

1/20 primer (5 pmol/µL)

5 µL

plasmid DNA (T2)

5 µL

Amount of primers and DNA sent for sequencing.

Primer

Code

prefix primer (forward)

17C4ZAD658

suffix primer (reverse)

17C4ZAD659

Sequencing codes Macrogen.

The results from Macrogen showed that T2 has the right sequence.

Harm Ruesink