Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Transformation (t33)
00:00, 9 June 2015 - 00:00, 9 June 2015
Afterwards competent E.coli was transformed with the ligation products. A heat shock transformation was used. The cultures were plated on LB+amp plates and incubated overnight at 37 °C. They were labeled as:
100% comp E. coli with 23 lig
100% comp E. coli with 24 lig
00:00, 10 June 2015 - 00:00, 10 June 2015
The transformation was checked. One white colony was visible on the 23 lig plate and 7 colonies were visible on the 24 lig plate. The colonies were grown overnight in LB+amp at 37 °C, 200 rpm.
BBa_K823023 + tasA (possible)
BBa_K823023 + RFP
BBa_K823024 + tasA 7x (possible)
BBa_K823024 + RFP