Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA ligation pSB1C3 (t41)
00:00, 8 July 2015 - 00:00, 8 July 2015
Afterwards tasA was ligated in pSB1C3.
Component
Amount
tasA DNA
10 µL
pSB1C3 DNA
5 µL
T4 ligase
0.5 µL
T4 ligase buffer
2 µL
\( \mathrm{H_2O}\)
2.5 µL
Components used for ligation.
The sample was ligated at room temperature for 2 hours.