Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA purification (t13)

00:00, 20 May 2015 - 00:00, 20 May 2015

The samples were digested for 2 hours at 37 °C. Afterwards the DNA was cleaned up with the PCR purification kit, and stored at -20 °C. The DNA was stored in 20 µL elution buffer.

Sample

Name

Plasmid

ligate RFP BB, digest prod 20-5-15

tasA

ligate tasA 4.2, digest prod 20-5-15

Storage information of purified PCR product.

NOTE: The next time a gel extraction must be done after digestion, as the RFP insert is too large to get rid of with the PCR purification kit.

Harm Ruesink