Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA sequencing (t38)

00:00, 18 June 2015 - 00:00, 18 June 2015

24TA 3, one of the tasA digestion samples, was send to Macrogen for sequencing:

5µL 1/20 primer (5 pmol/µL)

5µL plasmid DNA (24TA 3)

Sequencing code Macrogen:

Forward primer (5 tasA for): 1790ZAB067

Reverse primer (6 tasA rev): 1790ZAB068

Harm Ruesink

00:00, 19 June 2015 - 00:00, 19 June 2015

The seqencing results showed the tasA insert without restriction sites. The restriction sites are gone.

Harm Ruesink