Template:Team:Groningen/CONTENT/LOGBOOK/Backbone Digestion t24
Digestion of RFP backbone (t24)
Restriction of the RFP backbone and tasA DNA, in order to make the ligation possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The RFP backbone and tasA DNA were digested.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 30 April 2015 - 00:00, 30 April 2015
Three different possible backbones were grown overnight in LB+amp and LB+cm, of which one was the RFP backbone.