Template:Team:Groningen/CONTENT/LOGBOOK/Backbone Groningen

Backbone from previous iGEM Groningen team

Growing a backbone from a previous iGEM Groningen team.

To grow a backbone.

Partial success.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/No_protocol">None</a>

00:00, 30 April 2015 - 00:00, 30 April 2015

Grew 3 different possible backbones overnight in LB + AMP and LB + CM

Harm

00:00, 1 June 2015 - 00:00, 1 June 2015

1 grew in AMP, 2 and 3 grew in CM. 1 is E. coli and the other two are B. subtilis and have no plasmids.

Miniprep culture #1 (Thermo Scientific #K0502, lot: 00272723)

stored as: ‘RFP backbone’ in -20 °C (50 µL, 90 ng/µL DNA).

Harm