Template:Team:Groningen/CONTENT/LOGBOOK/Colony PCR pg16
Colony PCR (pg16)
A colony PCR was carried out to check if the ligation was successful.
Check if inserts have the correct size
No right insert was seen.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/ColonyPCR">Colony PCR</a>
12:00, 23 July 2015 - 12:00, 23 July 2015
One white colony visible on the kanamycin plate. A colony PCR was done with prefix and suffix primers. PGA is around 3000bp, so extention time has to be around 3:00 minutes. After PCR sample was loaded on gel to check insert. No insert was seen.