Template:Team:Groningen/CONTENT/LOGBOOK/Colony PCR pg27
Colony PCR (pg27)
A colony PCR was carried out to check if the insert
Check if insert has the correct size.
None of the colonies had the right insert.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/ColonyPCR">Colony PCR</a>
00:00, 3 August 2015 - 00:00, 3 August 2015
A few colonies were on the transformation plate from 31-7-15. Colony PCR was done with pSB1C3 primers. PCR product was checked on a 1% agarose gel with 1:50000 SERVA DNA Stain G. No bands were detected.