Template:Team:Groningen/CONTENT/LOGBOOK/Grow Backbone t24
Grow Backbone (t24)
Grow E. coli with plasmid for plasmid extraction for ligation with tasA.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
E. coli with plasmid was grown in LB. Bacillus did not have a plasmid.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/None">None</a>
00:00, 01 May 2015 - 00:00, 01 May 2015
The first E. coli backbone grew overnight with amp, the second and third backbones, which were Bacillus backbones, grew with cm.