Template:Team:Groningen/CONTENT/LOGBOOK/Ligation Backbone pg14
Ligation Backbone (pg14)
Ligate the combined part of PGA into pSB1C3 to get the DNA into a backbone.
Make a more negatively charged biofilm.
The ligation did not work according to the following colony PCRs
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
12:00, 22 July 2015 - 12:00, 22 July 2015
Ligation of PGA into pSB1C3 according to protocol.