Template:Team:Groningen/CONTENT/LOGBOOK/Ligation in pSB1C3 pg25
Ligation in pSB1C3 (pg25)
Ligate PGA into pSB1C3 backbone. This we did to be able to transform it into E. coli.
Make a more negatively charged biofilm.
Ligation failed as can be seen in colony PCR
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 31 July 2015 - 00:00, 31 July 2015
Ligation of PGA in pSB1C3.