Template:Team:Groningen/CONTENT/LOGBOOK/Ligation pg5
Ligation (pg5)
Ligation was done to combine the PGA DNA and a vector in order to transform it into E. coli.
Make a more negatively charged biofilm.
Ligation was not successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 15 July 2015 - 00:00, 15 July 2015
Ligated in an linearized pSB1C3 (digested with EcoRI and PstI)