Template:Team:Groningen/CONTENT/LOGBOOK/Ligation th16

Ligation thrC locus with thrC plasmid A (th16)
Ligation of thrC locus 2 with thrC plasmid A
Extra integration of DNA in thrC locus
Ligated thrC locus 2 with thrC plasmid A

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>

00:00, 3 September 2015 - 00:00, 3 September 2015
The thrC locus 2 was ligated with thrC plasmid A without AmyE locus 2. The ligation mixture was incubated for 1 hour at room temperature.
Marieke Mulder