Template:Team:Groningen/CONTENT/LOGBOOK/Potentials

Potentials
An experimental setup for the measurement of potential over (ion exchange) membranes is described.
To measure potential differences created by different kinds of membranes.
There was a measured potential with varying values over several biofilms.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/No_protocol">None</a>

00:00, 00 Month 2015 - 00:00, 00 Month 2015

Value
Date
Carrier
Biofilm
Growth
Concentration
Electrodes
Remarks
200-300 mV
10-7
Nylon
3610 comI
MSgg agar
30/1
Ag-AgCl B
Wrong Ag (sterling)
200-220 mV
10-7
Nylon
30/1
Ag-AgCl B
Wrong Ag (sterling)
180-200 mV
10-7
Nylon
30/1
Ag-AgCl B
Wrong Ag (sterling)
180-200 mV
10-7
Nylon
3610 comI
MSgg agar
30/30
Ag-AgCl B
Wrong Ag (sterling)
20 mV
20-7
Nylon
30/1
Com
20 mV
20-7
Nylon
30/1
Com
6 mV
20-7
W2
30/1
Com
18 mV
29-7
W2
comI
dfr
30/1
Com
18 mV
29-7
W2
Alginate
30/1
Com
18-20 mV
29-7
W2
Alginate
30/1
Com
33 mV
29-7
W2
Alginate
30/1
Com
36 mV
29-7
W2
Alginate
30/1
Com
17 mV
30-7
W2
comI
dfr
30/1
Com
dried out plate before dfr
19 mV
30-7
W2
comI
dfr
30/1
Com
dried out plate before dfr
19 mV
31-7
Nylon
comI
cfr MSgg H
30/1
Com
15 mV
5-8
Nylon
comI
sandwich
30/1
Com
13 mV
5-8
Nylon
sandwich
30/1
Com
14 mV
5-8
Nylon
sandwich
30/1
Com
7 mV
5-8
Nylon
30/1
Com
12 mV
5-8
Nylon
30/1
Com
12 mV
5-8
W2
30/1
Com
12.5 mV
5-8
W2
30/1
Com
15 mV
5-8
W4
30/1
Com
6 mV
5-8
W4
30/1
Com
17 mV
5-8
W6
30/1
Com
7-15 mV
5-8
W6
30/1
Com
-10 mV
5-8
W2
30/1
Com
-10 mV
4-9
W4
10/1
Com
-11 mV
4-9
W4
10/1
Com
-11 mV
4-9
W4
15/1
Com
-12 mV
4-9
W4
15/1
Com
-11.5 mV
4-9
W4
15/1
Com
-12 mV
4-9
W4
20/1
Com
-15 mV
4-9
W4
20/1
Com
-16 mV
4-9
W4
20/1
Com
-16.5 mV
4-9
W4
20/1
Com
-16.5 mV
4-9
W4
20/1
Com
-15 mV
4-9
W4
25/1
Com
-16 mV
4-9
W4
25/1
Com
-17 mV
4-9
W4
25/1
Com
-18.5 mV
4-9
W4
25/1
Com
-17.7 mV
4-9
W4
30/1
Com
-17.8 mV
4-9
W4
30/1
Com
-18 mV ⟶ -19 mV
4-9
W4
30/1
Com
-18 mV ⟶ -19 mV
4-9
W4
30/1
Com
-11 mV ⟶ -13 mV
5-9
W2
30/1
Com
-13 mV ⟶ -15 mV
5-9
W2
30/1
Com
-13 mV
5-9
W2
30/1
Com
-13 mV
5-9
W2
25/1
Com
-13 mV
5-9
W2
25/1
Com
-13 mV
5-9
W2
25/1
Com
-12.5 mV
5-9
W2
20/1
Com
-12 mV
5-9
W2
20/1
Com
-12 mV
5-9
W2
20/1
Com
-11 mV
5-9
W2
15/1
Com
-11 mV
5-9
W2
15/1
Com
-11 mV
5-9
W2
15/1
Com
-11 mV
5-9
W2
10/1
Com
-10 mV
5-9
W2
10/1
Com
-10 mV
5-9
W2
10/1
Com
+8 mV ⟶ -12 mV
5-9
W6
comI
MSgg Agar
30/1
Com
+6.5 mV ⟶ -8 mV
5-9
W4
JH642 bslA
MSgg Agar
30/1
Com
+0 mV ⟶ -8 mV
5-9
W4
JH642 bslA
MSgg Agar
30/1
Com
-14 mV
10-9
Coffee Filter
Natto
LB Agar
30/1
Com
Missed beginning
-8 mV
10-9
Coffee Filter
Natto
LB Agar
30/1
Com
2nd run, missed end
1.2 mV ⟶ -4.6 mV
10-9
Coffee Filter
Natto
LB Agar
30/1
Com
-10 mV
10-9
Coffee Filter
Natto abrB
LB Agar
30/1
Com
-10 mV
10-9
Coffee Filter
Natto abrB
LB Agar
30/1
Com
second run
-11 mV
10-9
Coffee Filter
Natto slrR
MSgg Agar
30/1
Com
-11 mV
10-9
Coffee Filter
Natto slrR
MSgg Agar
30/1
Com
Second run
-8 mV
10-9
Coffee Filter
Natto slrR
LB Agar
30/1
Com
Missed start
-8 mV
10-9
Coffee Filter
Natto slrR
LB Agar
30/1
Com
Second run
-8 mV ⟶ -2 mV ⟶ -8 mV
9-9
Coffee Filter
Natto sinR
MSgg Agar
30/1
Com
-8 mV
9-9
Coffee Filter
Natto sinR
MSgg Agar
30/1
Com
-8 mV
9-9
Coffee Filter
Natto abrB
MSgg Agar
30/1
Com
-9 mV
9-9
Coffee Filter
Natto abrB
MSgg Agar
30/1
Com
Second run
-5 mV ⟶ 0 ⟶ 2 mV
9-9
Coffee Filter
Natto bslA
MSgg Agar
30/1
Com
-11 mV
9-9
Coffee Filter
Natto bslA
MSgg Agar
30/1
Com
Second run
-6 mV
9-9
Coffee Filter
Natto
MSgg Agar
30/1
Com
Missed start
-5 mV
9-9
Coffee Filter
Natto
MSgg Agar
30/1
Com
Second run
-6 mV
9-9
Coffee Filter
Natto
MSgg Agar
30/1
Com
Missed start
-5 mV
9-9
Coffee Filter
Natto
MSgg Agar
30/1
Com
Second run
-5 mV
9-9
Coffee Filter
Natto bslA
LB Agar
30/1
Com
-2 mV
9-9
Coffee Filter
Natto bslA
LB Agar
30/1
Com
Second run
-5 mV
9-9
Coffee Filter
Natto sinR
LB Agar
30/1
Com
-7 mV
9-9
Coffee Filter
Natto sinR
LB Agar
30/1
Com
Second run
-10 mV
9-9
Coffee Filter
3610
30/1
Com
-10 mV
9-9
Coffee Filter
3610
LB Agar
30/1
Com
Second run
1.5 mV ⟶ -10 mV
14-9
W4
3610
30/1
Com
-12 ⟶ -14 mV
14-9
W4
3610
30/1
Com
Second run
0 ⟶ -10 mV
14-9
W4
3610
30/1
Com
-11 ⟶ -15 mV
14-9
W4
3610
30/1
Com
-10 ⟶ -12 mV
14-9
W4
36 tasA
30/1
Com
Second run
-6 ⟶ -4 mV
14-9
W4
36 tasA
30/1
Com
-3 ⟶ -15 mV
14-9
W4
36 tasA
30/1
Com
Second run
3 ⟶ -8 mV
14-9
W4
36 tasA
30/1
Com
-12 ⟶ -13 mV
14-9
W4
36 tasA
30/1
Com
Second run
6 ⟶ -8 mV
14-9
W4
36 bslA
30/1
Com
-14 mV
14-9
W4
36 bslA
30/1
Com
Second run
9 ⟶ -14 mV
14-9
W4
36 bslA
30/1
Com
-12 mV
14-9
W4
36 bslA
30/1
Com
Second run
7 ⟶ -6 mV
14-9
W4
36 bslA
30/1
Com
-11 mV
14-9
W4
36 bslA
30/1
Com
Second run
-6 ⟶ -12 mV
14-9
W4
36 abrB
30/1
Com
-14 mV
14-9
W4
36 bslA
30/1
Com
Second run
2 ⟶ -9 mV
14-9
W4
36 abrB
30/1
Com
-11 mV ⟶ -12 mV
14-9
W4
36 abrB
30/1
Com
Second run
2 mV ⟶ -9 mV
14-9
W4
36 abrB
30/1
Com
-11 mV ⟶ -12 mV
14-9
W4
36 abrB
30/1
Com
Second run
7 mV ⟶ -9 mV
14-9
W4
36 abrB
30/1
Com
Reversed Electrodes
-12V
14-9
W4
36 abrB
30/1
Com
Second run
0 mV ⟶ -13 mV
14-9
W4
36 slrR
30/1
Com
Torn carrier
-14 mV
14-9
W4
36 slrR
30/1
Com
Second run
6 mV ⟶ -7 mV
14-9
W4
36 slrR
30/1
Com
-13 mV
14-9
W4
36 slrR
30/1
Com
Second run
Potentials.

Timon van der Berg, Hanneke Siebe