From the colonies on the plate, 8 were taken and tested for their insert with a colony PCR. After PCR samples were loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results don't show the correct insert.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Colony PCR zp18
Psalt Colony PCR (zp18)
A colony PCR was performed to check the insert of a transformed E. coli.
To create a salt inducible promotor.
Gel results show not the correct insert.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/ColonyPCR">Colony PCR</a>
00:00, 15 July 2015 - 00:00, 15 July 2015